Journal: Nutrients

Article Title: Methanolic Extract of Cimicifuga foetida Induces G 1 Cell Cycle Arrest and Apoptosis and Inhibits Metastasis of Glioma Cells

doi: 10.3390/nu16193254

Figure Lengend Snippet: Effects of water (CF-WE) and methanol (CF-ME) extracts of Cimicifuga foetida on the viability of glioblastoma (GBM) and glial cells. ( A ) Cell viability of U87 MG, A172, and T98G GBM cell lines treated with varying concentrations (0–800 μg/mL) of CF-WE for 24, 48, and 72 h showed a significant dose- and time-dependent decrease in cell viability, with stronger effects at higher concentrations and longer treatment durations. ( B ) Cell viability of U87 MG, A172, and T98G GBM cell lines treated with CF-ME (0–150 μg/mL) for the same durations exhibited a stronger inhibitory effect, also in a dose- and time-dependent manner. ( C ) Cell viability of human normal glial cell line SVGp12 treated with CF-WE (left) and CF-ME (right) under similar conditions showed reduced cell viability, but SVGp12 cells exhibited higher overall viability and IC 50 values than GBM cells, indicating lower toxicity toward normal glial cells. * Asterisks indicate statistical significance compared to control (0 μg/mL) ( p < 0.05). Error bars represent the mean ± SD of three independent experiments.

Article Snippet: U87 MG, A172, and T98G (GBM cell lines) and SVGp12 (normal human glial cell line) were obtained from the Bioresource Collection and Research Center (BCRC).

Techniques: Control

Journal: Nutrients

Article Title: Methanolic Extract of Cimicifuga foetida Induces G 1 Cell Cycle Arrest and Apoptosis and Inhibits Metastasis of Glioma Cells

doi: 10.3390/nu16193254

Figure Lengend Snippet: IC 50 values of malignant glioma cells and normal glial cells after treatment with extract of Cimicifuga foetida . U87 MG, A172, T98G, and SVGp12 cells were treated with different doses of CF-WE and CF-ME for 72 h, respectively. Cell viability was detected using an MTT assay with an ELISA reader to measure absorbance values. IC 50 values were calculated after plotting the regression lines. Data are presented as means ± standard deviation.

Article Snippet: U87 MG, A172, and T98G (GBM cell lines) and SVGp12 (normal human glial cell line) were obtained from the Bioresource Collection and Research Center (BCRC).

Techniques: MTT Assay, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Condition Medium of Glioblastoma Cell Lines Decreases the Viability of Glioblastoma Cells by Modulating Gene Expression Profile

doi: 10.1101/2021.09.11.459916

Figure Lengend Snippet: Cellular viability and colony-forming ability were measured following the MTT and CFA protocols, respectively. a) Absorbance values obtain from SVG p12 cells by CMs of U87 and LN229, DMEM, and DMSO treatments. b) Representative images of formed SVG p12 colonies. c) Comparison of the number of formed SVG p12 colonies upon different treatments. Statistical significance was demonstrated with an asterisk. *p-value < 0.05, ***p-value < 0.001. ns; nonsignificant.

Article Snippet: Human likely glioblastoma cell lines LN229 and U87, and human astroglial cell line SVG p12 (American Type Culture Collection #Catalog numbers: CRL-2611 TM , HTB-14, CRL-8621; respectively), were used in this study.

Techniques: Comparison

Journal: bioRxiv

Article Title: Condition Medium of Glioblastoma Cell Lines Decreases the Viability of Glioblastoma Cells by Modulating Gene Expression Profile

doi: 10.1101/2021.09.11.459916

Figure Lengend Snippet: Alteration of cellular viability and colony formation capacity of U87 cells. a) U87 MTT results after treatment of LN229 and SVG p12 CMs, DMEM, and DMSO. b) Representative images of U87 colonies. c) Colony numbers of U87 cells formed after LN229 and SVG p12 CMs treatment. Statistical significance is demonstrated with an asterisk. *p-value < 0.05, **p-value < 0.01, and ***p-value < 0.001. ns; nonsignificant.

Article Snippet: Human likely glioblastoma cell lines LN229 and U87, and human astroglial cell line SVG p12 (American Type Culture Collection #Catalog numbers: CRL-2611 TM , HTB-14, CRL-8621; respectively), were used in this study.

Techniques: